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1 h pretreatment, 4 h/24 h tracer incubation) engages PHGDH in MDA-MB-468 cells, reducing glucose-derived serine synthesis, impairing one-carbon unit incorporation into nucleotides from both endogenous and exogenous serine, and increasing SHMT1-mediated one-carbon unit wasting

We therefore propose that these alterations potentially constitute an early-risk signature that continues to intensify, driving ferroptosis-dependent germ-cell clearance at PD3, a conclusion further corroborated by Fer-1 rescue experiments

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